Mass Spectrometry for Chemical Biology
Sabareesh is interested in applying mass spectrometric methods for investigating a variety of biomolecules. Biological processes and transformations are concurrent with alterations of chemical properties, implying molecular modifications. Therefore, characterization of biomolecules provides clues to comprehend biological events. Sensitivity and ability to directly investigate crude mixtures are a few merits that render mass spectrometry more useful than other techniques to study biomolecules. This includes application of different chromatographic procedures as well.
- A curious aspect is to find or develop suitable method(s) for the determination of primary structure of medium and large sized polypeptides (proteins).
- Identification of post-translational and/or covalent modifications in proteins.
- The above two aims automatically invokes the need for understanding gas phase fragmentation pathways of peptide or polypeptide molecular ions.
- Identifying N60D mutation in omega subunit of Escherichia coli RNA polymerase by bottom-up proteomic approach. Sabareesh V*, Sarkar P, Sardesai AA, Chatterji D. Analyst 2010, 135, 2723 - 2729.
- Peptide Fragment Ion Analyser (PFIA): A simple and versatile tool for the interpretation of tandem mass spectrometric data and de novo sequencing of peptides. Jagannath S, Sabareesh V* Rapid Commun. Mass Spectrom. 2007, 21, 3033-3038 (Download)
- Sequencing of T-superfamily conotoxins from Conus virgo: Pyroglutamic acid identification and disulfide arrangement by MALDI mass Spectrometry. Mandal AK, Ramasamy MRS, Sabareesh V, Openshaw ME, Krishnan KS, Balaram P. J. Am. Soc. Mass Spectrom. 2007, 18, 1396-1404.
- Identification and characterization of a library of microheterogeneous cyclohexadepsipeptides from the fungus Isaria. Sabareesh V, Ranganayaki RS, Raghothama S, Bopanna MP, Balaram H, Srinivasan MC, Balaram P. J. Nat. Prod. 2007, 70, 715-729.
- Mutations in hpyAVIBM, C5 cytosine DNA methyltransferase from Helicobacter pylori result in relaxed specificity. Kumar, R., Sabareesh, V., Mukhopadhyay, A.K., Rao, D.N. 2012. FEBS J. 279:1080-92.