Debojyoti Chakraborty

Senior Scientist
Genome Editing, Stem cells and Regenerative Medicine , Long noncoding RNAs

Our lab works on three broad research areas. The first of these is the development of efficient and specific tools for genome editing and lncRNA discovery. The second area is the systematic dissection of factors governing embryonic stem cell fate. The third area covers the use of cerebral organoids to address developmental questions such as influence of DNA topology on transcription.

Our major interests are
  • CRISPR/Cas9 based tools for genome editing and lncRNA discovery
  • Organoids for studying developmentally regulated lncRNAs within the context of chromatin architecture
  • Identification of novel determinants of embryonic stem cell fate

Using a combination of rational design of CRISPR Cas9 components and characterisation of novel Cas9 orthologs, we have identified methods to increase the efficiency and specificity of this genome editing system. We are engaged in protein engineering of a novel Cas9 protein to develop a compact and efficient tool for gene therapy. We have setup an efficient system for growing mouse 'mini brains' from embryonic stem cells in the petri dish and currently using this model to study lncRNA expression during different developmental stages. In a parallel study, we are understanding how pluripotency factors can regulate chromatin architecture in a cell cycle specific manner.

Selected Publications

Contact Information

Debojyoti Chakraborty
Office:
Room No. 129,
CSIR-IGIB,
Sukhdev Vihar,
Mathura Road,
New Delhi - 110 020
Tel: 91-11-29879 129

e-mail:
debojyoti.chakraborty@igib.in

Lab Webpage
Researchgate Profile

Linkout

Assistant Professor, Academy of Scientific and Innovative Research