Debojyoti Chakraborty

Senior Scientist
Genome editing, Stem cells and Organoid Biology

Our lab works on three broad research areas. The first of these is the development of efficient and specific tools for therapeutic genome editing, particularly for sickle cell anaemia. The second area is the systematic dissection of factors governing embryonic stem cell fate. The third area covers the use of cerebral organoids to address developmental questions such as influence of DNA topology on transcription.

Our major interests are
  • Development of CRISPR/Cas9 based tools for ex-vivo (iPSC) and in-vivo (base editing) therapeutic genome engineering
  • Studying determinants of brain development using cerebral organoids
  • Mechanistic understanding of novel determinants of embryonic stem cell fate

Using a combination of rational design of CRISPR Cas9 components and characterisation of novel Cas9 orthologs, we have identified methods to increase the efficiency and specificity of this genome editing system. We are engaged in protein engineering of an orthogonal Cas9 protein to develop a compact and efficient tool for gene therapy. Using a system for growing human and mouse 'mini brains' from embryonic stem cells or iPSCs we are studying how the mammalian brain develops and what regulates neuronal migration defects in certain disorders.

Selected Publications

Contact Information

Debojyoti Chakraborty
Room No. 129,
Sukhdev Vihar,
Mathura Road,
New Delhi - 110 020
Tel: 91-11-29879 129


Lab Webpage
Researchgate Profile


Assistant Professor, Academy of Scientific and Innovative Research