Santosh Kumar Upadhyay

Biomolecular NMR Spectroscopy - Structural Biology

Santosh Kumar Upadhyay is an NMR structural biophysicist, interested NMR spectroscopy of protein-nucleic acid complexes.

The current areas of research include:

  • Interactions between protein and nucleic acid using solution Nuclear Magnetic Resonance (NMR).
  • Biophysical (ITC, CD, Fluorescence etc) and biochemical methods for studying binding, structural and conformational changes and dynamics of proteins and nucleic acids.

The p53 tumor suppressor gene plays important role in preventing cancer development. Inactivation or loss of p53 tumor suppressor gene is the cause of most of the human cancers. RNA-binding proteins (RBPs) have important role in modulating p53 activity and its downstream targets. Several RBPs either promote or inhibit p53 translation. We are using a combined approach including structural and functional techniques. Nuclear magnetic resonance (NMR) spectroscopy is one of the powerful method available to study biomolecular structure and dynamics at atomic resolution in solution. NMR data offers insights into three-dimensional fold of proteins and their interactions with other biomolecules. The dynamics of conformational changes in protein complexes underlie many enzymatic reactions thereby allowing specificity. Our current focus is to elucidate structural and functional aspects of the molecular interactions between RNA binding proteins involved in RNA processing, especially alternative splicing and p21 mRNA, a downstream target of p53

Selected Publications:

Contact Information

Santosh Kumar Upadhyay

Sukhdev Vihar, 
Mathura Road, 
New Delhi - 110 025


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